Format

Send to

Choose Destination
Prog Mol Biol Transl Sci. 2009;85:91-135. doi: 10.1016/S0079-6603(08)00803-9.

Endonucleolytic initiation of mRNA decay in Escherichia coli.

Author information

1
Laboratoire de Microbiologie et Génétique Moléculaires, CNRS et Université Paul Sabatier, 31062 Toulouse, France.

Abstract

Instability is a fundamental property of mRNA that is necessary for the regulation of gene expression. In E. coli, the turnover of mRNA involves multiple, redundant pathways involving 3'-exoribonucleases, endoribonucleases, and a variety of other enzymes that modify RNA covalently or affect its conformation. Endoribonucleases are thought to initiate or accelerate the process of mRNA degradation. A major endoribonuclease in this process is RNase E, which is a key component of the degradative machinery amongst the Proteobacteria. RNase E is the central element in a multienzyme complex known as the RNA degradosome. Structural and functional data are converging on models for the mechanism of activation and regulation of RNase E and its paralog, RNase G. Here, we discuss current models for mRNA degradation in E. coli and we present current thinking on the structure and function of RNase E based on recent crystal structures of its catalytic core.

PMID:
19215771
DOI:
10.1016/S0079-6603(08)00803-9
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center