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Appl Biochem Biotechnol. 2010 Jan;160(1):232-43. doi: 10.1007/s12010-009-8540-0. Epub 2009 Feb 12.

Genetic engineering of Caulobacter crescentus for removal of cadmium from water.

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Department of Biological Sciences, Bowling Green State University, Bowling Green, OH 43403-0208, USA.


Hexa-histidine (6His) peptide was inserted to a permissive site of the surface layer (S-layer) protein RsaA of Caulobacter crescentus. The recombinant strain JS4022/p723-6H, expressing RsaA-6His fusion protein was examined for its ability to sequester Cd(II) from the bacterial growth medium. When mixed with 1 ppm CdCl(2), JS4022/p723-6H removed 94.3 approximately 99.9% of the Cd(II), whereas the control strain removed only 11.4 approximately 37.0%, depending on experimental conditions. The effective contact time of the cells and Cd(II) was as short as 15 min. When higher concentrations of CdCl(2) were tested, JS4022/p723-6H consistently demonstrated enhanced binding capacity over the control strain. At 15 ppm of Cd(II), each gram of JS4022/p723-6H dry cells retrieved 16.0 mg of Cd(II), comparing to 11.6 mg g(-1) achieved by the control strain. This work provides a potential cost-effective solution toward bioremediation of heavy metals from aqueous systems.

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