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J Clin Immunol. 2009 Jul;29(4):517-23. doi: 10.1007/s10875-009-9276-x. Epub 2009 Feb 13.

Increased IgG antibody-induced cytotoxicity against airway epithelial cells in patients with nonallergic asthma.

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1
Department of Allergy and Rheumatology, Ajou University School of Medicine, Suwon, South Korea.

Abstract

BACKGROUND:

IgG autoantibodies to airway epithelial cell proteins have been detected in patients with nonallergic asthma.

OBJECTIVE AND METHODS:

To evaluate the functional significance of these autoantibodies, we examined the presence of IgG antibody-induced cytotoxicity against airway epithelial cells (A549) by the microcytotoxicity assay using IgG antibodies purified from patients with nonallergic asthma.

RESULTS:

IgG antibody-induced cytotoxicity (expressed as percent cell lysis) was significantly increased in nine patients with nonallergic asthma (mean +/- standard deviation; 30.6 +/- 7.3%) as compared with eight healthy controls (13.9 +/- 5.1%) and nine patients with allergic asthma (20.3 +/- 10.4%; p < 0.05). In addition, IgG antibody-induced cytotoxicity was significantly inhibited when IgG antibodies from patients with nonallergic asthma were pre-incubated with recombinant human airway epithelial cell autoantigens (cytokeratin 18 or alpha-enolase proteins; p < 0.05).

CONCLUSION:

These results suggest a possible involvement of IgG autoantibody-induced cytotoxicity against airway epithelial cells in the pathogenesis of nonallergic asthma.

PMID:
19214721
DOI:
10.1007/s10875-009-9276-x
[Indexed for MEDLINE]
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