Format

Send to

Choose Destination
See comment in PubMed Commons below
Immunol Res. 2009 Dec;45(2-3):218-28. doi: 10.1007/s12026-009-8103-z. Epub 2009 Feb 11.

An approach to the identification of T cell epitopes in the genomic era: application to Francisella tularensis.

Author information

1
Department of Microbiology and Immunology, University of Rochester Medical Center, NY 14642, USA.

Abstract

The identification and characterization of epitopes is essential for modern immunologic studies. Here, we describe a novel methodology we have developed to identify T cell epitopes exploiting the phenomenon of cross presentation. Particulate antigens, in the form of beads, are very effective in delivering exogenous antigen to both the class I and class II pathways. We will review our efforts to screen entire genomes of pathogens for T cell epitopes taking advantage of the advances in genomics using Francisella tularensis as a model. By automating aspects of this technology we will be able to functionally screen the entire genome of F. tularensis for T cell epitopes. This technology should be applicable not only to F. tularensis, but also to many other pathogens as well.

PMID:
19212707
PMCID:
PMC4976928
DOI:
10.1007/s12026-009-8103-z
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Springer Icon for PubMed Central
    Loading ...
    Support Center