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Lab Chip. 2009 Jan 7;9(1):167-70. doi: 10.1039/b810341k. Epub 2008 Oct 20.

Direct rapid prototyping of PDMS from a photomask film for micropatterning of biomolecules and cells.

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Department of Bio and Brain Engineering, KAIST, Yuseong-gu, Daejeon 305-701, Korea.


The soft lithographic technique is a collection of simple and cost-effective patterning techniques which applies an elastomeric stamp to transfer a nano/micro-scale pattern. Patterning biological materials using soft lithography provides procedurally simple control of the surface chemistry and the cell environments. However, conventional methods for generating microstructures on a substrate require expensive clean room facilities and skillful training. Here we report a simple and inexpensive clean-room free process using a conventional photomask film as a master to fabricate elastomeric stamps or microfluidic channels. This ultra rapid prototyping technique was applied to print FITC labeled poly-L-lysine with a 10 microm feature size on a glass substrate using soft lithographic processes, such as micro-contact printing and micromolding in capillaries, for patterning human hepatocellular carcinoma cells, human skin fibroblasts and hippocampal neurons from E-18 Sprague-Dawley rat. This novel technique using a photomask film as a master would be very useful 'hands-on' tool for the generation of micro-patterned chemical or biological assays using cells and proteins.

[Indexed for MEDLINE]

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