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J Am Chem Soc. 2009 Feb 25;131(7):2484-6. doi: 10.1021/ja8086938.

Protein nanoparticles engineered to sense kinase activity in MRI.

Author information

1
Department of Biological Engineering, Massachusetts Institute of Technology, 150 Albany Street, NW14-2213, Cambridge, Massachusetts 02139, USA.

Abstract

We introduce a family of protein nanoparticles capable of sensing analytes in conjunction with magnetic resonance imaging (MRI). The new sensors are derived from the iron storage protein ferritin (Ft); they are designed and optimized using facile protein engineering methods, and self-assembled in cells harboring specific combinations of DNA coding sequences. As illustration, we show that suitably constructed Ft-based sensors can report activity of the important neural signaling enzyme protein kinase A (PKA). Phosphorylation of the engineered Ft-based nanoparticles by PKA promotes clustering and changes in T(2)-weighted MRI signal.

PMID:
19199639
PMCID:
PMC2662502
DOI:
10.1021/ja8086938
[Indexed for MEDLINE]
Free PMC Article

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