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Nat Methods. 2009 Mar;6(3):199-201. doi: 10.1038/nmeth.1301. Epub 2009 Feb 8.

Direct determination of haplotypes from single DNA molecules.

Author information

1
Cardiovascular Research Institute, University of California-San Francisco, 513 Parnassus Ave., San Francisco, CA 94143, USA. mingx@bionanomatrix.com

Abstract

Determining the long-range haplotypes in a diploid individual is a major technical challenge. Here we report a method of molecular haplotyping by directly imaging multiple polymorphic sites on individual human DNA molecules simultaneously. We demonstrate the utility of this technology by accurately determining the haplotypes consisting of up to 16 single-nucleotide polymorphisms in genomic regions up to 50 kilobases.

PMID:
19198595
PMCID:
PMC3880790
DOI:
10.1038/nmeth.1301
[Indexed for MEDLINE]
Free PMC Article

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