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Nat Methods. 2009 Mar;6(3):203-5. doi: 10.1038/nmeth.1302. Epub 2009 Feb 8.

Quantitative interaction proteomics using mass spectrometry.

Author information

1
Institute of Molecular Systems Biology, Swiss Federal Institute of Technology, Eidgenössische Technische Hochschule Zürich, Wolfgang Pauli-Strasse 16, Zurich, Switzerland.

Abstract

We present a mass spectrometry-based strategy for the absolute quantification of protein complex components isolated through affinity purification. We quantified bait proteins via isotope-labeled reference peptides corresponding to an affinity tag sequence and prey proteins by label-free correlational quantification using the precursor ion signal intensities of proteotypic peptides generated in reciprocal purifications. We used this method to quantitatively analyze interaction stoichiometries in the human protein phosphatase 2A network.

PMID:
19198594
DOI:
10.1038/nmeth.1302
[Indexed for MEDLINE]

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