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J Gen Virol. 1991 Oct;72 ( Pt 10):2563-7.

Characterization of the VPg-dsRNA linkage of infectious pancreatic necrosis virus.

Author information

1
Department of Microbiology, College of Biological Sciences, University of Guelph, Ontario, Canada.

Abstract

By the use of strong denaturing agents, a genome-linked protein (VPg)-RNA complex was purified from infectious pancreatic necrosis virus. Ribonuclease treatment of 125I-labelled VPg-RNA released a 90K polypeptide identical to the minor structural polypeptide VP1 (the putative RNA polymerase), as determined by peptide mapping. The polypeptide is linked to the RNA by a serine-5' GMP phosphodiester bond. The results identify birnaviruses as the only dsRNA viruses with a VPg, the size of which is the largest of the VPgs of RNA viruses.

PMID:
1919532
DOI:
10.1099/0022-1317-72-10-2563
[Indexed for MEDLINE]

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