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J Chromatogr A. 2009 Mar 20;1216(12):2449-55. doi: 10.1016/j.chroma.2009.01.031. Epub 2009 Jan 17.

Development and validation of a liquid chromatographic method for the analysis of capreomycin sulfate and its related substances.

Author information

1
Laboratory for Pharmaceutical Analysis, KU.Leuven, O&N II, Leuven, Belgium.

Abstract

A gradient LC method for the analysis of capreomycin sulfate and its related substances was developed. The chromatographic conditions include the use of a Hypersil base deactivated C(18) (250 mm x 4.6mm, 5 microm) column maintained at 25 degrees C, a mobile phase containing acetonitrile, phosphate buffer pH 2.3 and 0.025M hexanesulfonate at a flow rate of 1.0 mL/min and UV detection performed at 268 nm. Good separation of the four active components of capreomycin and eleven unknown impurities was achieved. A system suitability test to check the quality of the separation is specified. The method shows good repeatability, linearity and robustness.

PMID:
19185869
DOI:
10.1016/j.chroma.2009.01.031
[Indexed for MEDLINE]

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