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J Am Soc Mass Spectrom. 2009 Apr;20(4):652-7. doi: 10.1016/j.jasms.2008.12.002. Epub 2008 Dec 9.

Differentiation of 3-O-sulfated heparin disaccharide isomers: identification of structural aspects of the heparin CCL2 binding motif.

Author information

1
Department of Molecular and Cellular Biology, University of California, Davis, California 95616, USA.

Abstract

The presence of 3-O-sulfated glucosamine residues in heparin or heparan sulfate plays a role in binding to antithrombin III and HSV infection. In this study, tandem mass spectrometry was used to differentiate between two heparin disaccharide isomers containing variable sulfate at C6 in a common disaccharide and C3 in a more rare one. The dissociation patterns shown by MS(2) and MS(3) were clearly distinguishable between the isomers, allowing their differentiation and quantitation. Using this technique, we show that an octasaccharide with 11 sulfate groups with high affinity for inflammatory chemokine CCL2 does not contain 3-O-sulfated disaccharides.

PMID:
19185514
PMCID:
PMC2758813
DOI:
10.1016/j.jasms.2008.12.002
[Indexed for MEDLINE]
Free PMC Article

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