Abstract
The Mycobacterium tuberculosis cmk gene, predicted to encode a CMP kinase (CMK), was cloned and expressed, and its product was purified to homogeneity. Steady-state kinetics confirmed that M. tuberculosis CMK is a monomer that preferentially phosphorylates CMP and dCMP by a sequential mechanism. A plausible role for CMK is discussed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Cloning, Molecular
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Deoxycytidine Monophosphate / metabolism*
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Gene Expression
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Kinetics
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Molecular Sequence Data
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Mycobacterium tuberculosis / enzymology*
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Mycobacterium tuberculosis / genetics*
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Nucleoside-Phosphate Kinase / genetics*
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Nucleoside-Phosphate Kinase / metabolism*
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Phosphorylation
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / metabolism
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Sequence Alignment
Substances
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Recombinant Proteins
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Deoxycytidine Monophosphate
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cytidylate kinase
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Nucleoside-Phosphate Kinase