Retinal histology and ERG of NHR mice following suppression of RHO. Fellow eyes from NHR (RHO+/−Rho−/−) mice were injected with 2 µl 2 × 1012 of vp/ml AAVshQ1 and AAVshNT at postnatal day 10 and analyzed 4 weeks postinjection. For histology, eyes (n = 3) were fixed in 4% paraformaldehyde and cryosectioned (12 µm). Cy3 label RHO immunocytochemistry was carried out and nuclei counterstained with DAPI. Representative microscopic images are provided from eyes injected with AAVshNT (a, d, and g), AAVshQ1 (b, e, and h), and from uninjected eyes (c, f, and i). Red, green, and blue fluorescence corresponds to signals of RHO, EGFP, and cell nuclei, respectively. For ERG analysis (n = 6), mice were dark adapted over night and ERG responses recorded from both eyes. (j) Means of relative amplitudes of ERGs are given for rod-isolated (rod), mixed rod and cone (mixed), and cone-isolated (cone) responses corresponding to AAVshNT (NT) and AAVshQ1 (Q1) injections. OS, photoreceptor outer segments; IS, photoreceptor inner segments; ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer; Bar = 25 µm. Error bars correspond to SD values; *P < 0.05.