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Nat Methods. 2009 Feb;6(2):131-3. doi: 10.1038/nmeth.1296. Epub 2009 Jan 25.

A bright and photostable photoconvertible fluorescent protein.

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1
Howard Hughes Medical Institute, Janelia Farm Research Campus, 19700 Helix Drive, Ashburn, Virginia 20147, USA.

Abstract

Photoconvertible fluorescent proteins are potential tools for investigating dynamic processes in living cells and for emerging super-resolution microscopy techniques. Unfortunately, most probes in this class are hampered by oligomerization, small photon budgets or poor photostability. Here we report an EosFP variant that functions well in a broad range of protein fusions for dynamic investigations, exhibits high photostability and preserves the approximately 10-nm localization precision of its parent.

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PMID:
19169260
PMCID:
PMC2745648
DOI:
10.1038/nmeth.1296
[Indexed for MEDLINE]
Free PMC Article

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