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Vaccine. 2009 Mar 18;27(13):1974-83. doi: 10.1016/j.vaccine.2009.01.008. Epub 2009 Jan 23.

Attenuation of coxsackievirus B3 by VP2 mutation and its application as a vaccine against virus-induced myocarditis and pancreatitis.

Author information

1
Department of Biotechnology, The Catholic University of Korea, Bucheon 420-743, Republic of Korea.

Abstract

Coxsackievirus B3 (CVB3) is a common agent of viral myocarditis, a major cause of sudden cardiac death, and ultimately dilated cardiomyopathy. However, there is no vaccine in clinical use. In this study, we identified the conserved amino acid sequences in the C-terminal region of the VP2 of the coxsackievirus B group and some echoviruses. The mutant virus, YYFF, with phenylalanines substituted for two tyrosines in these conserved sequences was highly attenuated in vivo and could induce a high neutralizing antibody titer and a cytotoxic T-lymphocyte response against CVB3. Thereby, mutant-virus-immunized mice showed a 100% survival rate and protection against inflammation of the heart and pancreas after lethal dose challenge. Thus, this mutant virus is a good candidate for an attenuated CVB3 vaccine.

PMID:
19168108
DOI:
10.1016/j.vaccine.2009.01.008
[Indexed for MEDLINE]

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