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Virology. 2009 Mar 15;385(2):383-91. doi: 10.1016/j.virol.2008.12.029. Epub 2009 Jan 21.

Vaccinia virus exhibits cell-type-dependent entry characteristics.

Author information

1
School of Dental Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA. whitbeck@biochem.dental.upenn.edu

Abstract

Differing and sometimes conflicting data have been reported regarding several aspects of vaccinia virus (VV) entry. To address this, we used a beta-galactosidase reporter virus to monitor virus entry into multiple cell types under varying conditions. Entry into HeLa, B78H1 and L cells was strongly inhibited by heparin whereas entry into Vero and BSC-1 cells was unaffected. Bafilomycin also exhibited variable and cell-type-specific effects on VV entry. Entry into B78H1 and BSC-1 cells was strongly inhibited by bafilomycin whereas entry into Vero and HeLa cells was only partially inhibited suggesting the co-existence of both pH-dependent and pH-independent VV entry pathways in these cell types. Finally, entry into HeLa, B78H1, L and BSC-1 cells exhibited a lag of 6-9 min whereas this delay was undetectable in Vero cells. Our results suggest that VV exploits multiple cell attachment and entry pathways allowing it to infect a broad range of cells.

PMID:
19162290
PMCID:
PMC4041486
DOI:
10.1016/j.virol.2008.12.029
[Indexed for MEDLINE]
Free PMC Article

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