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Methods Enzymol. 2008;447:521-35. doi: 10.1016/S0076-6879(08)02225-8.

RNA decay by messenger RNA interferases.

Author information

1
Institute for Cell and Molecular Biosciences, Medical School, University of Newcastle, Newcastle, United Kingdom.

Abstract

Two abundant toxin-antitoxin (TA) gene families, relBE and mazEF, encode mRNA cleaving enzymes whose ectopic overexpression abruptly inhibits translation and thereby induces a bacteriostatic condition. Here we describe and discuss protocols for the overproduction, purification, and analysis of mRNA cleaving enzymes such as RelE of Escherichia coli and the corresponding antitoxin RelB. In particular, we describe a set of plasmid vectors useful for the detailed analysis of cleavage sites in model mRNAs.

PMID:
19161859
DOI:
10.1016/S0076-6879(08)02225-8
[Indexed for MEDLINE]

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