A differentially expressed maize gene has been cloned and sequenced. Transcriptional and translational start sites have been mapped and 2.5 kb of 5' flanking DNA were sequenced. The 8 kDa protein encoded by this gene shows striking similarity to the metallothionein-like proteins recently described in Pisum sativum and Mimulus guttatus. The maize MT-L gene message is very abundant in roots without exposure to high levels of metals, present at lower concentration in leaves and pith, and at very low concentration in seed.