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Angew Chem Int Ed Engl. 2009;48(9):1633-5. doi: 10.1002/anie.200805420.

A pyrrolysine analogue for protein click chemistry.

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1
Department of Chemistry, The Ohio State University, 484 West 12th Avenue, Columbus, OH 43210, USA.

Abstract

Ignoring the STOP sign: A pyrrolysine analogue bearing a terminal alkyne was site-specifically incorporated into recombinant calmodulin (CaM) through a UAG codon. The resulting protein was labeled with an azide-containing dye using a copper(I)-catalyzed click reaction. Subsequent application of an orthogonal cysteine tagging method yielded a CaM labeled with two distinct fluorophores that enabled its study by FRET spectroscopy.

PMID:
19156778
DOI:
10.1002/anie.200805420
[Indexed for MEDLINE]
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