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Methods Mol Biol. 2009;499:175-94. doi: 10.1007/978-1-60327-151-6_17.

Large-scale gene disruption using the UAU1 cassette.

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  • 1Department of Microbiology Columbia University, NY, USA.

Abstract

Candida albicans is a major fungal systemic pathogen in humans. Genetic manipulation of C. albicans is unwieldy. We report here a strategy that is useful and successful for large-scale genetic manipulation of C. albicans genes of interest: use of the UAU1 cassette on a Tn7 transposon. Streamlined yet admittedly flawed disruption techniques, such as the one described here, may prove vital to uncovering the genetic basis of fungal virulence.

PMID:
19152049
PMCID:
PMC2786983
DOI:
10.1007/978-1-60327-151-6_17
[PubMed - indexed for MEDLINE]
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