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Nat Methods. 2009 Feb;6(2):143-5. doi: 10.1038/nmeth.1295. Epub 2009 Jan 18.

In vivo imaging of extracellular matrix remodeling by tumor-associated fibroblasts.

Author information

1
Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, 100 Blossom Street, Boston, Massachusetts 02114, USA.

Abstract

Here we integrated multiphoton laser scanning microscopy and the registration of second harmonic generation images of collagen fibers to overcome difficulties in tracking stromal cell-matrix interactions for several days in live mice. We show that the matrix-modifying hormone relaxin increased tumor-associated fibroblast (TAF) interaction with collagen fibers by stimulating beta1-integrin activity, which is necessary for fiber remodeling by matrix metalloproteinases.

PMID:
19151720
PMCID:
PMC2877598
DOI:
10.1038/nmeth.1295
[Indexed for MEDLINE]
Free PMC Article

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