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Mol Biochem Parasitol. 2009 Apr;164(2):131-6. doi: 10.1016/j.molbiopara.2008.12.008. Epub 2008 Dec 27.

Hydroxyurea-induced synchronisation of bloodstream stage Trypanosoma brucei.

Author information

1
Division of Infection & Immunity and Wellcome Centre for Molecular Parasitology, Glasgow Biomedical Research Centre, University of Glasgow, 120 University Place, Glasgow, Scotland, UK.

Abstract

Synchronisation of the Trypanosoma brucei cell cycle proved elusive for many years. A recent report demonstrated that synchronisation of procyclic form cells was possible following treatment with hydroxyurea. Here, that work is extended to the disease-relevant, mammalian-infective bloodstream stage trypanosome. Treatment of bloodstream stage Lister 427 T. brucei cells growing in vitro with 10 microg ml(-1) hydroxyurea for 6h led to an enrichment of cells in S phase. Following removal of the drug, cells proceeded uniformly through one round of the cell cycle, providing a much needed tool to enrich for specific cell cycle stages, in a manner similar to hydroxyurea treatment of procyclic form T. brucei.

PMID:
19150633
PMCID:
PMC6218013
DOI:
10.1016/j.molbiopara.2008.12.008
[Indexed for MEDLINE]
Free PMC Article

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