Format

Send to

Choose Destination
J Microbiol Methods. 2009 Mar;76(3):320-3. doi: 10.1016/j.mimet.2008.12.007. Epub 2008 Dec 29.

Facile construction of unmarked deletion mutants in Burkholderia pseudomallei using sacB counter-selection in sucrose-resistant and sucrose-sensitive isolates.

Author information

1
Institute for Glycomics, Griffith University, Gold Coast Campus, Queensland 4222, Australia.

Abstract

Burkholderia pseudomallei is the causative agent of melioidosis, a potentially fatal disease endemic or emerging world-wide. Here we report unmarked allele-replacement mutagenesis using efficient sacB counter-selection. Despite being genotypically sacB(+), most commonly used B. pseudomallei strains are sucrose-resistant and efficient sacB counter-selection is demonstrated in both resistant and sensitive strains.

PMID:
19150470
DOI:
10.1016/j.mimet.2008.12.007
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center