J Am Chem Soc. 2009 Feb 11;131(5):1644-5. doi: 10.1021/ja807391g.

Selective recognition of unnatural imidazopyridopyrimidine:naphthyridine base pairs consisting of four hydrogen bonds by the Klenow fragment.

Minakawa N¹, Ogata S, Takahashi M, Matsuda A.

Author information

1: Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.

Abstract

In this work, we investigated how thermally stable ImO(N):NaN(O) and ImN(O):NaO(N) pairs are recognized by the Klenow fragment (KF). As a result, these complementary base pairs, especially the ImN(O):NaO(N) pair, were recognized selectively due to the four hydrogen bonds between the nucleobases and the shape complementarity of the Im:Na pair similar to the purine:pyrimidine base pair.