Send to

Choose Destination
See comment in PubMed Commons below
Acta Biomater. 2009 Jun;5(5):1725-31. doi: 10.1016/j.actbio.2008.12.006. Epub 2008 Dec 25.

Preparation of porous bioactive ceramic microspheres and in vitro osteoblastic culturing for tissue engineering application.

Author information

  • 1Department of Biomaterials Science, School of Dentistry, Dankook University, Cheonan, 330-714, South Korea.


Microparticulates are useful for directly filling defective tissues as well as for delivering cells and bioactive molecules in regenerative medicine. This paper reports on the production of bioactive ceramic microspheres with an interconnected macropore structure. The sol-gel derived calcium silicate powder was homogenized with an oligomeric Camphene melt, which was used as a novel porogen, and spherical-shaped microparticulates were obtained by an oil-in-water emulsion method. A porous structure was generated through the sublimation of Camphene within the calcium silicate-Camphene solidified blend under ambient conditions. The microspheres retained the crystalline phase of apatite and wollastonite during heat treatment and induced calcium phosphate precipitation under a body-simulating medium, showing the characteristics of bone-bioactive materials. Osteoblastic cells were observed to anchor to and spread well over the surface of the porous microspheres, and further to proliferate actively with culturing time. The bioactive and porous microspheres developed are considered potentially useful in the regeneration of hard tissues as a matrix for tissue engineering as well as a direct filling material.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center