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Contraception. 2009 Feb;79(2):139-45. doi: 10.1016/j.contraception.2008.08.008. Epub 2008 Oct 9.

Effects of progesterone, levonorgestrel and medroxyprogesterone acetate on apoptosis in human endometrial endothelial cells.

Author information

1
CONRAD Clinical Research Center, Department of Obstetrics and Gynecology, Eastern Virginia Medical School, Norfolk, VA 23507, USA.

Abstract

BACKGROUND:

We evaluated apoptosis in human endometrial endothelial cells (HEECs) incubated with progesterone, levonorgestrel (LNG) and medroxyprogesterone acetate (MPA).

STUDY DESIGN:

HEECs were cultured to near confluence, and the progestogens were added.

SETTING:

Academic Department of Obstetrics and Gynecology.

PATIENTS:

No patients were involved.

INTERVENTIONS:

Progestogens at 5-, 250- and 500-ng/mL concentrations were added to incubations of HEECs for 12, 24 and 48 h.

MAIN OUTCOME MEASURE:

Apoptosis based on terminal deoxynucleotidyl transferase-mediated deoxy-UTP nick-end labeling (TUNEL), and semiquantification of Bax and Bcl-2.

RESULTS:

No apoptosis was found by TUNEL, Bax and Bcl-2 after 12 h incubation with any progestogen. TUNEL increased after incubation for 24 and 48 h with progesterone 500 ng/mL; LNG 250, 500 ng/mL and all concentrations of MPA (p<.001), Bax increased and Bcl-2 decreased at all concentrations of MPA and the two highest concentrations of LNG at 48 h (p<.05).

CONCLUSION:

MPA results in apoptosis of HEECs.

[Indexed for MEDLINE]

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