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Biophys J. 2009 Jan;96(1):L01-3. doi: 10.1016/j.bpj.2008.09.032.

In vivo labeling method using a genetic construct for nanoscale resolution microscopy.

Author information

1
Leica Microsystems CMS, Mannheim, Germany. jan.schroeder@leica-microsystems.com

Abstract

We demonstrate beam scanning-stimulated emission depletion microscopy with in vivo labeled cells. A red emitting fluorescent dye is introduced into membrane protein fused to a multifunctional reporter protein (HaloTag, Promega, Madison, WI) in live cells. This approach allows superresolution stimulated emission depletion imaging without the limitations of immunofluorescence-based staining.

PMID:
19134467
PMCID:
PMC2710050
DOI:
10.1016/j.bpj.2008.09.032
[Indexed for MEDLINE]
Free PMC Article
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