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J Proteome Res. 2009 Feb;8(2):1030-6. doi: 10.1021/pr800832s.

Prion disease diagnosis by proteomic profiling.

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1
Department of Comparative Biosciences, University of Wisconsin-Madison, Wisconsin 53705-222, USA.

Abstract

Definitive prion disease diagnosis is currently limited to postmortem assay for the presence of the disease-associated proteinase K-resistant prion protein. Using cerebrospinal fluid (CSF) from prion-infected hamsters, matrix-assisted laser desorption/ionization Fourier transform mass spectrometry (MALDI-FTMS), and support vector machines (SVM), we have identified peptide profiles characteristic of disease state. Using 10-fold leave-one-out cross-validation, we report a predictive accuracy of 72% with a true positive rate of 73% and a false positive rate of 27% demonstrating the suitability of using proteomic profiling and CSF for the development of multiple marker diagnostics of prion disease.

PMID:
19133784
PMCID:
PMC2652651
DOI:
10.1021/pr800832s
[Indexed for MEDLINE]
Free PMC Article
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