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J Insect Physiol. 2009 Mar;55(3):264-72. doi: 10.1016/j.jinsphys.2008.12.008. Epub 2009 Jan 22.

Proteomic analysis of the mosquito Aedes aegypti midgut brush border membrane vesicles.

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  • 1Department of Biochemistry, The Ohio State University, 484 West 12th Avenue, Columbus, OH 43210, USA.

Abstract

We analyzed brush border membrane vesicle proteins from isolated midguts of the mosquito Aedes aegypti, by two proteomic methods: two-dimensional gel electrophoresis (isoelectric focusing and SDS-PAGE) and a shotgun two-dimensional liquid chromatographic (LS/LS) approach based on multidimensional protein identification technology (MudPIT). We were interested in the most abundant proteins of the apical brush border midgut membrane. About 400 spots were detected on 2D gels and 39 spots were cored and identified by mass spectrometry. 86 proteins were identified by MudPIT. Three proteins, arginine kinase, putative allergen and actin are shown to be the most predominant proteins in the sample. The total number of 36 proteins detected by both methods represents the most abundant proteins in the BBMV.

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