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Clin Chim Acta. 2009 Mar;401(1-2):128-33. doi: 10.1016/j.cca.2008.12.008. Epub 2008 Dec 14.

Upregulation and secretion of macrophage inhibitory cytokine-1 (MIC-1) in gastric cancers.

Author information

1
Stem Cell Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea.

Abstract

BACKGROUND:

Macrophage inhibitory cytokine-1 (MIC-1), a distant member of the transforming growth factor (TGF)-beta superfamily, has been reported to be upregulated and secreted from several cancers. We examined MIC-1 expression and secretion in gastric cancers.

METHODS:

MIC-1 mRNA and protein levels in cancer tissues and cell lines were analyzed by RT-PCR and Western blot. MIC-1 expression in cancer tissues and its secretion in serum were analyzed using immunohistochemistry and ELISA.

RESULTS:

MIC-1 was significantly upregulated in gastric cancer tissues and cell lines. MIC-1 was secreted from gastric SNU620 cells and its levels in the serum of cancer patients were 10-fold higher than those of healthy controls. In addition, the staining of MIC-1 expression was strongly increased in metastatic gastric cancers.

CONCLUSIONS:

MIC-1 was obviously overexpressed in gastric cancers and MIC-1 secretion into blood may be useful for the prediction of gastric cancer progression.

PMID:
19133249
DOI:
10.1016/j.cca.2008.12.008
[Indexed for MEDLINE]

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