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Agric Biol Chem. 1990;54(2):505-9.

Isolation and properties of a chromosome-dependent KHR killer toxin in Saccharomyces cerevisiae.

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National Research Institute of Brewing, 2-6-30 Takinogawa, Kita-ku, Tokyo 114, Japan.


A strain of the yeast Saccharomyces cerevisiae coding for KHR on the chromosome secreted a toxin that kills sensitive yeasts. The transformants of multicopy vectors carrying the KHR gene could secrete 3-4-fold the killer toxin of the donor strain. This toxic substance was purified 80-fold in specific activity from the culture filtrate by gel filtration and hydrophobic column chromatography. The purified toxin gave a single protein band with molecular mass of 20 kDa on SDS-PAGE and had an isoelectric point of pH 5.3. The toxin had novel killer activity against Candida glabrata and S. cerevisiae, but did not affect bacteria, fungi, or other yeasts.

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