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Biomed Microdevices. 2009 Jun;11(3):547-55. doi: 10.1007/s10544-008-9260-x.

Integrated microfluidic devices for combinatorial cell-based assays.

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1
Department of Mechanical and Aerospace Engineering, University of California, Los Angeles, CA 90095, USA.

Abstract

The development of miniaturized cell culture platforms for performing parallel cultures and combinatorial assays is important in cell biology from the single-cell level to the system level. In this paper we developed an integrated microfluidic cell-culture platform, Cell-microChip (Cell-microChip), for parallel analyses of the effects of microenvironmental cues (i.e., culture scaffolds) on different mammalian cells and their cellular responses to external stimuli. As a model study, we demonstrated the ability of culturing and assaying several mammalian cells, such as NIH 3T3 fibroblast, B16 melanoma and HeLa cell lines, in a parallel way. For functional assays, first we tested drug-induced apoptotic responses from different cell lines. As a second functional assay, we performed "on-chip" transfection of a reporter gene encoding an enhanced green fluorescent protein (EGFP) followed by live-cell imaging of transcriptional activation of cyclooxygenase 2 (Cox-2) expression. Collectively, our Cell-microChip approach demonstrated the capability to carry out parallel operations and the potential to further integrate advanced functions and applications in the broader space of combinatorial chemistry and biology.

PMID:
19130244
PMCID:
PMC2893875
DOI:
10.1007/s10544-008-9260-x
[Indexed for MEDLINE]
Free PMC Article
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