Melting curve analysis for rapid detection of topoisomerase gene mutations in Haemophilus influenzae

J Clin Microbiol. 2009 Mar;47(3):781-4. doi: 10.1128/JCM.01645-08. Epub 2009 Jan 7.

Abstract

We established a real-time PCR assay with melting curve analysis to rapidly genotype quinolone resistance-determining regions (QRDRs) of gyrase A and topoisomerase IV genes in Haemophilus influenzae. This assay is a useful tool for the detection of fluoroquinolone resistance and for the early detection of preexisting QRDR mutations.

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Bacterial Proteins / genetics
  • DNA Gyrase / genetics
  • DNA Topoisomerase IV / genetics*
  • DNA, Bacterial / genetics*
  • Drug Resistance, Bacterial
  • Haemophilus influenzae / enzymology*
  • Haemophilus influenzae / genetics*
  • Humans
  • Microbial Sensitivity Tests / methods
  • Mutation*
  • Polymerase Chain Reaction / methods
  • Quinolones / pharmacology
  • Transition Temperature*

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • DNA, Bacterial
  • Quinolones
  • DNA Topoisomerase IV
  • DNA Gyrase