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Fen Zi Xi Bao Sheng Wu Xue Bao. 2008 Oct;41(5):341-8.

[Knockdown and overexpression of miR-219 lead to embryonic defects in zebrafish development].

[Article in Chinese]

Author information

1
Institute of Molecular and Chemical Biology, East China Normal University, Shanghai 200062.

Abstract

MicroRNAs (miRNAs) are important post-transcriptional modulators of gene expression, which have been implicated in many physiological and pathological processes. In this study, we found that miR-219 expressed since early segmentation stages (16s) in spinal cord and mid-, hindbrain in zebrafish using Northern blotting and whole mounts ISH technologies. Moreover, knockdown or overexpression of miR-219 led to specific embryonic defects. Furthermore, TUNEL assay showed that overexpression of miR-219 induced significant cell apoptosis in the head and tail of zebrafish. Our study suggested that miR-219 may play an important role in zebrafish embryonic development.

PMID:
19127769
[Indexed for MEDLINE]

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