Format

Send to

Choose Destination
See comment in PubMed Commons below
Rev Sci Instrum. 2008 Dec;79(12):123707. doi: 10.1063/1.3043439.

Microbeam-integrated multiphoton imaging system.

Author information

  • 1Center for Radiological Research, Columbia University, New York, New York 10032, USA.

Abstract

Multiphoton microscopy has been added to the array of imaging techniques at the endstation for the Microbeam II cell irradiator at Columbia University's Radiological Research Accelerator Facility (RARAF). This three-dimensional (3D), laser-scanning microscope functions through multiphoton excitation, providing an enhanced imaging routine during radiation experiments with tissuelike samples, such as small living animals and organisms. Studies at RARAF focus on radiation effects; hence, this multiphoton microscope was designed to observe postirradiation cellular dynamics. This multiphoton microscope was custom designed into an existing Nikon Eclipse E600-FN research fluorescence microscope on the irradiation platform. Design details and biology applications using this enhanced 3D-imaging technique at RARAF are reviewed.

PMID:
19123569
PMCID:
PMC2736647
DOI:
10.1063/1.3043439
[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for American Institute of Physics Icon for PubMed Central
    Loading ...
    Support Center