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Methods Cell Biol. 2008;89:391-407. doi: 10.1016/S0091-679X(08)00614-6.

Chapter 14: Electron microscopy of hydrated samples.

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1
Whitehead Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

Abstract

Conventional electron microscopy offers a substantial resolution advantage over light microscopy, but requires difficult and often destructive preparation techniques. Recent advances in electron microscopy allow for imaging of hydrated samples, retaining the resolution advantage while removing the difficulty in preparation. Two new techniques, environmental scanning electron microscopy and wet electron microscopy offer this advantage, allowing for new possibilities in biological imaging.

PMID:
19118683
DOI:
10.1016/S0091-679X(08)00614-6
[Indexed for MEDLINE]

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