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J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Jan 15;877(3):213-20. doi: 10.1016/j.jchromb.2008.12.010. Epub 2008 Dec 11.

Quantitative high-performance liquid chromatography analysis of the pool levels of undecaprenyl phosphate and its derivatives in bacterial membranes.

Author information

1
Université Paris-Sud, UMR 8619, Institut de Biochimie et Biophysique Moléculaire et Cellulaire, 91405 Orsay Cedex, France. helene.barreteau@u-psud.fr

Abstract

Undecaprenyl phosphate is the essential lipid involved in the transport of hydrophilic motifs across the bacterial membranes during the synthesis of cell wall polymers such as peptidoglycan. A HPLC procedure was developed for the quantification of undecaprenyl phosphate and its two derivatives, undecaprenyl pyrophosphate and undecaprenol. During the exponential growth phase, the pools of undecaprenyl phosphate and undecaprenyl pyrophosphate were ca. 75 and 270 nmol/g of cell dry weight, respectively, in Escherichia coli, and ca. 50 and 150 nmol/g, respectively, in Staphylococcus aureus. Undecaprenol was detected in S. aureus (70 nmol/g), but not in E. coli (<1 nmol/g).

PMID:
19110475
DOI:
10.1016/j.jchromb.2008.12.010
[Indexed for MEDLINE]

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