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BMC Microbiol. 2008 Dec 23;8:233. doi: 10.1186/1471-2180-8-233.

Comparison of the MicroScan, VITEK 2, and Crystal GP with 16S rRNA sequencing and MicroSeq 500 v2.0 analysis for coagulase-negative Staphylococci.

Author information

1
Department of Laboratory Medicine, Seoul National University Hospital, 101 Daehang-no, Jongno-gu, Seoul, South Korea. rabbit790622@gmail.com

Abstract

BACKGROUND:

Three phenotypic identification systems (MicroScan, VITEK 2, and Crystal GP) were evaluated for their accuracy to identify coagulase-negative staphylococci (CNS). A total of 120 clinical isolates confirmed to be CNS via 16S rRNA sequencing and analysis with the MicroSeq 500 v2.0 database were assessed.

RESULTS:

The MicroScan, VITEK 2, and Crystal GP systems correctly identified 82.5%, 87.5%, and 67.5% of the isolates, respectively. Misidentification was the main problem in MicroScan (10.8%) and Crystal GP (23.3%) systems, whereas the main problem of VITEK 2 was low-level discrimination (7.5%).

CONCLUSION:

None of the 3 phenotypic systems tested could accurately and reliably identify CNS at the species level. Further verifications such as biochemical testing or 16S rRNA sequencing together with analysis using a comparable database might be helpful in this regard.

PMID:
19105808
PMCID:
PMC2633347
DOI:
10.1186/1471-2180-8-233
[Indexed for MEDLINE]
Free PMC Article

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