Format

Send to

Choose Destination
See comment in PubMed Commons below
Opt Express. 2008 Dec 22;16(26):21843-8.

Real-time extended depth of field microscopy.

Author information

1
Department of Engineering Science, University of Oxford, Parks Road, Oxford, OX1 3PJ, United Kingdom.

Abstract

We describe an optical microscope system whose focal setting can be changed quickly without moving the objective lens or specimen. Using this system, diffraction limited images can be acquired from a wide range of focal settings without introducing optical aberrations that degrade image quality. We combine this system with a real time Nipkow disc based confocal microscope so as to permit the acquisition of extended depth of field images directly in a single frame of the CCD camera. We also demonstrate a simple modification that enables extended depth of field images to be acquired from different angles of perspective, where the angle can be changed over a continuous range by the user in real-time.

PMID:
19104617
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments

    Supplemental Content

    Full text links

    Icon for Optical Society of America
    Loading ...
    Support Center