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Methods Mol Biol. 2008;468:99-110. doi: 10.1007/978-1-59745-249-6_8.

Assaying beta-catenin/TCF transcription with beta-catenin/TCF transcription-based reporter constructs.

Author information

1
Howard Hughes Medical Institute and Department of Pharmacology, University of Washington School of Medicine, Seattle, WA, USA.

Abstract

Transcription-based reporters have been instrumental in characterizing the Wnt/beta-catenin signaling pathway and will be essential in the search for therapeutics aimed at combating diseases linked to aberrant signaling. In this chapter, we introduce a new improved Wnt/beta-catenin reporter system, beta-catenin-activated reporter (BAR), and its accompanying control reporter system, found unresponsive BAR (fuBAR). Its enhanced sensitivity, increased dynamic range, and lentiviral platform provide a reporter system that will keep pace with the needs of scientists in the field.

PMID:
19099249
DOI:
10.1007/978-1-59745-249-6_8
[Indexed for MEDLINE]

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