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PLoS One. 2008;3(12):e3991. doi: 10.1371/journal.pone.0003991. Epub 2008 Dec 22.

Pasteurellaceae ComE1 proteins combine the properties of fibronectin adhesins and DNA binding competence proteins.

Author information

1
Division of Microbial Diseases, UCL Eastman Dental Institute, University College London, London, United Kingdom. l.mullen@eastman.ucl.ac.uk

Abstract

A novel fibronectin-binding protein from Pasteurella multocida (PM1665) that binds to the fibronectin type III(9-10) modules via two helix-hairpin-helix motifs has recently been described [1]. This protein shares homology with competence-related DNA-binding and uptake proteins (ComEA and ComE) from Gram-positive and Gram-negative bacteria. Here, we show that recombinant PM1665 (now designated ComE1) also binds to DNA through the same helix-hairpin-helix motifs required for fibronectin-binding. This binding to DNA is non sequence-specific and is confined to double-stranded DNA. We have cloned and expressed ComE1 proteins from five members of the Pasteurellaceae in order to further investigate the function(s) of these proteins. When expressed as recombinant GST-fusion proteins, all of the homologues bound both to fibronectin and to double-stranded DNA. Inactivation of the gene encoding the ComE1 homologue in Actinobacillus pleuropneumoniae indicates major roles for these proteins in at least two processes: natural transformation, and binding of bacteria to fibronectin.

PMID:
19098981
PMCID:
PMC2601033
DOI:
10.1371/journal.pone.0003991
[Indexed for MEDLINE]
Free PMC Article

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