Format

Send to

Choose Destination
Cell Cycle. 2009 Jan 1;8(1):167-71. Epub 2009 Jan 11.

APC/C- and Mad2-mediated degradation of Cdc20 during spindle checkpoint activation.

Author information

1
Department of Pathology, NYU Cancer Institute, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA.

Abstract

The spindle assembly checkpoint (SAC) is an important mechanism that prevents the separation of sister chromatids until the microtubules radiating from the spindle poles are correctly attached to the kinetochores. Cdc20, an activator of the Anaphase Promoting Complex/Cyclosome (APC/C), is known as a major downstream target for inhibition by the SAC through the binding of mitotic checkpoint proteins, such as Mad2 and BubR1. Here, we report that the SAC negatively regulates the stability of Cdc20 by targeting it for proteasome-dependent degradation. Once the checkpoint is activated by spindle poisons, a major population of Cdc20 is degraded via APC/C, an event that requires the binding of Cdc20 to Mad2. We propose that the degradation of Cdc20 represents a critical control mechanism to ensure inactivation of APC/C(Cdc20) in response to the SAC.

Comment in

PMID:
19098431
PMCID:
PMC2703714
DOI:
10.4161/cc.8.1.7606
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Taylor & Francis Icon for PubMed Central
Loading ...
Support Center