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Appl Environ Microbiol. 2009 Feb;75(4):1211-4. doi: 10.1128/AEM.02139-08. Epub 2008 Dec 19.

Cytosine deaminase as a negative selection marker for gene disruption and replacement in the genus Streptomyces and other actinobacteria.

Author information

1
Centre d'Etude et de Valorisation de la Diversité Microbienne, Département de Biologie, Faculté des Sciences, Université de Sherbrooke, Sherbrooke, QC J1K 2R1, Canada.

Abstract

We developed a novel negative selection system for actinobacteria based on cytosine deaminase (CodA). We constructed vectors that include a synthetic gene encoding the CodA protein from Escherichia coli optimized for expression in Streptomyces species. Gene disruption and the introduction of an unmarked in-frame deletion were successfully achieved with these vectors.

PMID:
19098221
PMCID:
PMC2643560
DOI:
10.1128/AEM.02139-08
[Indexed for MEDLINE]
Free PMC Article

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