Format

Send to

Choose Destination
Science. 2009 Jan 2;323(5910):101-6. doi: 10.1126/science.1163732. Epub 2008 Dec 18.

A glucosinolate metabolism pathway in living plant cells mediates broad-spectrum antifungal defense.

Author information

1
Department of Plant Microbe Interactions, Max Planck Institut für Züchtungsforschung, Carl-von-Linné-Weg 10, D-50829 Köln, Germany. bednarek@mpiz-koeln.mpg.de

Abstract

Selection pressure exerted by insects and microorganisms shapes the diversity of plant secondary metabolites. We identified a metabolic pathway for glucosinolates, known insect deterrents, that differs from the pathway activated by chewing insects. This pathway is active in living plant cells, may contribute to glucosinolate turnover, and has been recruited for broad-spectrum antifungal defense responses. The Arabidopsis CYP81F2 gene encodes a P450 monooxygenase that is essential for the pathogen-induced accumulation of 4-methoxyindol-3-ylmethylglucosinolate, which in turn is activated by the atypical PEN2 myrosinase (a type of beta-thioglucoside glucohydrolase) for antifungal defense. We propose that reiterated enzymatic cycles, controlling the generation of toxic molecules and their detoxification, enable the recruitment of glucosinolates in defense responses.

PMID:
19095900
DOI:
10.1126/science.1163732
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center