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Biochem Biophys Res Commun. 1991 Aug 30;179(1):401-6.

Evidence that the signal-initiating membrane protein CD9 is associated with small GTP-binding proteins.

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Department of Medicine, Cross Cancer Institute, Edmonton, Alberta, Canada.


F(ab')2 fragments of anti-CD9 mAb aggregate platelets by a thromboxane-dependent pathway implicating CD9 as signal initiating molecule. We demonstrate that mAbs directed against CD9, but not against GPIIb/IIIa specifically immunoprecipitate, from detergent lysates of human platelets, proteins of 25 and 26 kDa which bind [alpha 32P]GTP on nitrocellulose transfers. The binding is specific since it is blocked by GTP, but not by ATP. The GTP-binding proteins do not belong to a Mg(2+)-sensitive subset since they are unaffected by the addition of 2 microM-20 mM Mg2+. The observations demonstrate that CD9 is associated with selected small G-proteins.

[Indexed for MEDLINE]

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