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Reprod Sci. 2009 Jan;16(1):71-9. doi: 10.1177/1933719108324892. Epub 2008 Dec 15.

Role of protein kinase Calpha in regulation of [Ca2+](I) and force in human myometrium.

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Department of Biological Sciences, University of Delaware, Newark, Delaware 19716, USA.


Recent findings implicate protein kinase C in regulation of contraction of uterine muscle (myometrium). However, the role of protein kinase C isoforms in myometrial contraction remains uncertain. Therefore, this study examined protein kinase Calpha's role in regulation of contraction and intracellular calcium concentration ([Ca2+](I)) of myometrium from term pregnant women. The authors demonstrated that protein kinase Calpha inhibitor Go6976 decreased the amplitude of potassium chloride-induced myometrial contractions in a time-dependent manner. The treatment of the myometrial strips with protein kinase Calpha-specific antisense oligodeoxynucleotides decreased the potassium chloride-induced contraction and [Ca2+](I) response to 39.3% + 6.8% and 50.0% + 3.3%, respectively, compared to control. The sense oligonucleotides treatment did not significantly change the potassium chloride responses (89.8% + 6.8% and 93.9% + 4.5% of the control for the contraction and [Ca2+](I), respectively). These data, coupled with the observation that protein kinase Calpha levels are elevated in the pregnant myometrium, suggest the involvement of protein kinase Calpha in regulation of human uterine contraction.

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