Abstract
Epigallocatechin gallate (EGCG) is a major green tea polyphenol with pronounced antioxidative activity. The effects of EGCG on lifespan and stress resistance in wild-type N2 and transgenic strains of Caenorhabditis elegans [ HSP-16.2/GFP, MEV-1(KN1), FEM-1(HC17)] were investigated. The expression of HSP-16.2 (induced by the pro-oxidant juglone) and the intracellular levels of H (2)O (2) were inhibited by EGCG treatment. Daily administration of 220 muM EGCG increased the mean lifespan by 10.14 % and 14.27 % in N2 and FEM-1(HC17) strains, respectively, and 55 muM EGCG increased the mean lifespan in MEV-1(KN1) by 16.11 %. The survival rate was also increased under lethal oxidative stress by 65.05 %. These findings suggest that the increased mean lifespan and stress resistance in C. ELEGANS apparently depend, among other factors, on the antioxidant properties of EGCG.
MeSH terms
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Animals
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Antioxidants / pharmacology*
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Caenorhabditis / drug effects*
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Caenorhabditis / growth & development
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Caenorhabditis / metabolism
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Caenorhabditis elegans Proteins / antagonists & inhibitors
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Caenorhabditis elegans Proteins / metabolism
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Camellia sinensis*
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Catechin / analogs & derivatives*
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Catechin / pharmacology
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Free Radical Scavengers / pharmacology*
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Genes, Reporter / physiology
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Heat-Shock Proteins / antagonists & inhibitors
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Heat-Shock Proteins / metabolism
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Hydrogen Peroxide / antagonists & inhibitors
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Hydrogen Peroxide / metabolism
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Longevity
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Oxidative Stress / drug effects
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Plant Extracts / pharmacology*
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Superoxides / antagonists & inhibitors
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Tea
Substances
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Antioxidants
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Caenorhabditis elegans Proteins
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Free Radical Scavengers
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Heat-Shock Proteins
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Plant Extracts
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Tea
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hsp-16.2 protein, C elegans
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Superoxides
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Catechin
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Hydrogen Peroxide
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epigallocatechin gallate