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Methods Mol Biol. 2009;495:53-60. doi: 10.1007/978-1-59745-477-3_5.

Measurement of abscisic acid and gibberellins by gas chromatography/mass spectrometry.

Author information

1
Growth Regulation Research Group, RIKEN Plant Science Center, Tsurumi, Yokohama, Japan.

Abstract

Gas chromatography-mass spectrometry (GC-MS)-based analysis is an accurate and sensitive method to quantify plant hormones. This method is commonly used for analysis of low-molecular-weight compounds, such as abscisic acid (ABA), gibberellins (GAs), auxins, and brassinosteroids. Procedures are composed of four major steps: extraction, fractionation, derivatization, and detection. Here, we describe a protocol for quantification of ABA and GAs.

PMID:
19085147
DOI:
10.1007/978-1-59745-477-3_5
[Indexed for MEDLINE]

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