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Chem Commun (Camb). 2009 Jan 7;(1):34-46. doi: 10.1039/b813732c. Epub 2008 Oct 17.

Enzyme assays.

Author information

1
Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, Berne, 3012, Switzerland. jean-louis.reymond@ioc.unibe.ch

Abstract

Enzyme assays are analytical tools to visualize enzyme activities. In recent years a large variety of enzyme assays have been developed to assist the discovery and optimization of industrial enzymes, in particular for "white biotechnology" where selective enzymes are used with great success for economically viable, mild and environmentally benign production processes. The present article highlights the aspects of fluorogenic and chromogenic substrates, sensors, and enzyme fingerprinting, which are our particular areas of interest.

PMID:
19081993
DOI:
10.1039/b813732c
[Indexed for MEDLINE]

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