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Dev Cell. 2008 Dec;15(6):901-12. doi: 10.1016/j.devcel.2008.09.018.

The conserved protein SZY-20 opposes the Plk4-related kinase ZYG-1 to limit centrosome size.

Author information

1
Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20894, USA. mihyesong@mail.nih.gov

Abstract

Microtubules are organized by the centrosome, a dynamic organelle that exhibits changes in both size and number during the cell cycle. Here we show that SZY-20, a putative RNA-binding protein, plays a critical role in limiting centrosome size in C. elegans. SZY-20 localizes in part to centrosomes and in its absence centrosomes possess increased levels of centriolar and pericentriolar components including gamma-tubulin and the centriole duplication factors ZYG-1 and SPD-2. These enlarged centrosomes possess normal centrioles, nucleate more microtubules, and fail to properly direct a number of microtubule-dependent processes. Depletion of ZYG-1 restores normal centrosome size and function to szy-20 mutants, whereas loss of szy-20 suppresses the centrosome duplication defects in both zyg-1 and spd-2 mutants. Our results describe a pathway that determines centrosome size and implicate centriole duplication factors in this process.

PMID:
19081077
PMCID:
PMC2829447
DOI:
10.1016/j.devcel.2008.09.018
[Indexed for MEDLINE]
Free PMC Article

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